Detailed Notes on high performance liquid chromatography
Detailed Notes on high performance liquid chromatography
Blog Article
物質の持つ特定波長の光を吸収する性質を利用した検出器。次のようなものが存在している。
ディテクターから出力された、電気信号を記録し、そこからピークを検出、解釈を行う。結果は、感熱紙等に印字される。装置のコントロールをしないのであれば、どのメーカーの物を使用しても問題はないが、通常は、装置のコントロールも同時に行うため、同じメーカーの物を選択する。
This web page won't exist within your chosen language. Your desire was saved and you'll be notified once a page can be viewed inside your language.
Bubbling an inert gas in the cell phase releases unstable dissolved gases. This method known as sparging.
Various solvents have varying polarities, which impact their conversation Together with the stationary stage and in the end have an affect on the separation of analytes. Typical solvents Utilized in HPLC involve:
이러한 특징으로 고성능 액체 크로마토그래피는 전 세계 모든 과학 분야 및 산업의 기반을 뒷받침하는 과학기술로서의 위치를 확립하고 있습니다.
ⅱ. 액체 크로마토그래피 정보에 대해 더 자세한 내용은 크로마토그래피 학습센터를 참고해주세요.
測定時間は測定物質および測定パラメータによって大きく変動するが、一般的には数分から数十分/回程度である。
Altering the cell period’s composition as the separation progresses is one particular Answer to this problem. For your reversed-section separation we use an First mobile phase that is definitely a lot more polar. As being click here the separation progresses, we adjust the composition of cellular period to ensure that it turns into considerably less polar (see Figure 12.five.six
The a few purple circles are binary cell phases produced by combining equal volumes of your pure cell phases. The ternary cellular phase revealed because of the purple get more info circle is made up of all a few of the pure cellular phases.
Modifying the cell section’s polarity index alterations a solute’s retention element. As we discovered in Chapter 12.three, nevertheless, a modify in k is not a powerful way to further improve resolution once the initial value of k is greater than ten.
The selection to begin with acetonitrile is arbitrary—we can easily just as simply opt for to start with methanol or with tetrahydrofuran.
are developed by reacting the silica particles with an organochlorosilane of the final kind Si(CH3)2RCl, exactly where R is surely an alkyl or substituted alkyl group.
Two problems usually shorten the life time of an analytical column. Initially, solutes that bind irreversibly on the stationary phase degrade the column’s performance by reducing the amount of stationary period available for effecting a separation. 2nd, particulate material injected While using the sample could clog the analytical column.